Effect of Ovarian Stimulation Protocol on Embryo Quality in IVF-ICSI

Abstract

Background:Defects in reproduction that may involve both the male & femalepartners result in the inability to procreate which is known as infertility. Theuse of assisted reproductive technologies (ART’s) has been increasing over thepast three decades. Many efforts have been done to compensate for unsatisfactoryresults in in-vitro fertilization (IVF) procedures, so that patients undergodifferent ovarian stimulation protocols to control retrieval of multiple oocytesin a single cycle. Although ovarian stimulation has an important role in ART’s,it may also have detrimental effects on oocyte quality, embryo quality, and IVFoutcomes.Objectives: To assess the effect of long agonist versus antagonist protocols onoocyte and embryo quality in IVF- Intracytoplasmic sperm injection (ICSI).Patients,Materials and Methods:This comparative study was done on the embryos of seventy one infertile femaleswho were undergoing Intracytoplasmic sperm injection (ICSI) treatmentregardless to the presence or absence of previous trials. These patients weredivided into two groups, 45 of them treated with agonist protocol and other 26patients treated with antagonist protocol. First oocyte retrieved was used forfurther assessment. Of the 71 oocytes, there were eight immature oocytes (3 atgerminal vesicle (GV) stage, 5 at metaphase I (MI) stage) and three abnormaloocytes, and these were excluded from the following ICSI treatment. Assessmentand comparison of oocyte maturity, fertilization rate, cleavage rate and resultingembryo quality were done.Results:The results of the present study showed significant difference (p<0.05)in oocyte number and maturity but no significant (p>0.05) difference betweenthe group treated with long agonist and those on antagonist protocol regardingfertilization rate, cleavage rate, and embryo quality.Conclusions:The type of protocol used in in-vitro fertilization has no detrimentaleffect on oocyte or embryo quality.