The Association of TSHRGene rs2268458 Polymorphism with Hypothyroidism in Females of Babylon Province-Iraq

Abstract

The Aim:To evaluate the association of TSHR gene polymorphism rs2268458with some Physio-biochemical parameters among hypothyroidism women. Methods: This studyincluded 51 hypothyroidism women and43 healthy women as a control group. Some Physio-biochemical (body mass index (BMI),cytochrome 450 1A1 (CYP1A1), calcium,and phosphorus) and hormonal assay (Osteopontin(OPN),thyroid stimulating hormone (TSH), fT3, fT4 and Calcitonin(CT)) assay were performed. Genotypingof rs-2268458 of TSHRgene was carried out using the polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) technique.Results:TSHR rs2268458 was associated with hypothyroidism in Iraqi women and allele C can be a risk factor for disease by OR=4.393,CI=1.42-13.53whereTCandCCgenotypes were associated withhigh BMI, while TC genotype was associated with high calcitonin and CYP1A1 levels.Conclusion:TheTSHRrs2268458 polymorphismwas associated with hypothyroidism in Iraqi women and allele C can be a risk factor for some Physio-biochemical and hormonal disorder in hypothyroidism women.