Genotyping of Pseudomonas Aeruginosa Isolated from Different Clinical Samples by Using ERIC Method

Abstract

This study included 100 clinical samples collected as following: 33, 27, 15, 14 and 11 samples from patients with otitis media, burn infections, urinary tract infections, wound infections, and bacteremia, respectively, during the period from September to December, 2014. Seventy-five Pseudomonas aeruginosa isolates were identified. To determine the genetic relatedness of different isolates, Pseudomonas aeruginosa has been typed using Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) and dendrogram analysis. Results showed that there was a genetic relatedness among Pseudomonas aeruginosa isolates in 19 clones, while 8 isolates contained different genotyping. In conclusion. The results inducted that ERIC-PCR is a practical, useful and easy method for typing Pseudomonas aeruginosa isolates.