EFFECT OF SODIUM CHLORIDE ON THE GROWTH OF EMBRYOGENIC CALLUS FOR DATE PALM (BARHI CULTIVAR) AFTER CRYOPRESERVATIVE

Abstract

The current study was conducted for the period from October 2014 until July 2017 in order to increase the susceptibility of date palm )phoenix dactylifera L(. to withstand the water stress using plant tissue culture and treated with some materials used for cryopreservative , The embryonic callus from the cultivating of the Shoot tips, planted on the Murashige and Skoog media, was subjected to immersion in preservative solution (Glycerol, PEG and DMSO) and cultivation on a sucrose-containing medium (342.3 g.L-1) for seven days at 27ºC in the dark, to save the embryonic callus in liquid nitrogen at -196ºC for 6 and 12 weeks, The callus was cultured on media included sodium chloride at concentrations 0, 1500, 3000 and 4500 ppm. The effect of sodium chloride on the growth rate and fresh weight of embryonic callus and number of embryos. The results showed that the effect of tri-interactions between sodium chloride and preservative solutions and concentrations was significant in increasing the number of embryos. The interaction treatment between sodium chloride 1500 ppm, DMSO and 10% concentration was excelled by recording it as the highest number of embryos was 23.2 embryo. The triple interaction treatment between 3000 ppm sodium chloride, DMSO, 10% concentration has excelled by giving it the highest growth rate 0.0372 mg.day-1, while the interaction treatment between 4500 ppm sodium chloride, PEG and 10% concentration recording the lowest growth rate 0.0002 mg.day-1. The interaction treatment between control and sodium chloride with its three concentrations, DMSO, PEG and 20% concentration didn't recorded any growth rate.