Detection of clfA gene in Staphylococcus aureus isolated from tonsillitis patients


Abstract Staphylococcus aureus is an opportunistic pathogen and it was one of virulent causer of tonsillitis. The present investigation was aimed to the molecular detection of adhesion gene (clfA) was done by polymerase chain reaction (PCR) and DNA sequencing. Only 64 isolates (42%) were mannitol fermenter and recorded as S. aureus, these isolates recovered from 152 swabs were collected from tonsillitis patients in ENT department in Al–Habboby Teaching Hospital, Thi-Qar province, during the period from November 2016 to March 2017. Out of 64 isolates, 56 (87.5%) were harbored clfA gene. The sequencing of PCR products showed significant alignments identities (96-99%) to the S. aureus which are located in BLAST-NCBI Genbank. Phylogenetic analysis of S. aureus based upon the neighbour-joining of partial clfA gene sequences showed that these sequences were derived from Staphylococcus genes.