INCIDENCE AND ANTOBIOTIC SUSCEPTIBILITY PATTERNS OF PSEUDOMONAS AERUGINOSA IN BURNS INFECTION IN DUHOK CITY

Abstract

Background and objectives Pseudomonas aeruginosa is an opportunistic pathogen causing severe, acute and chronic nosocomial infections. The organism is generally resistant to numerous antimicrobial agents, making the treatment of infections further difficult. The aim of this study was to determine the incidence of Pseudomonas aeruginosa involved in burns infections. Also, the susceptibility and resistotyping patterns of the isolates to commonlyprescribed antibiotics were studied.Methods During a period of six months between July and December, 2010, a total of 159samples from burns infections, using sterile cotton swabs, were collected from Burns Hospital in Duhok city. The samples were plated on Blood agar and MacConkey agar and the isolates were identified by routine procedures. Antibiotics susceptibility and resistantprofiles to 14 commonly prescribed antibiotics were performed by the disc diffusion method using Mueller-Hinton agar.Results Out of the 159 samples collected from burns infections, 116 samples were showed bacterial growth, 76 (47.7%) were Pseudomonas aeruginosa, followed by Klebsiella pneumoniae 20 (12.5%), Escherichia coli 10 (6.2%), Staphylococcus aureus 8 (5 %),Staphylococcus epidermidis 2 (1.2%), and no growth 43 (27 %). The results showed that the occurrence of Pseudomonas aeruginosa was higher than the other groups of bacteria. The sensitivity pattern of Pseudomonas aeruginosa revealed that the organism was highly sensitive to impenem (98.6%) followed by piperacillin (60.5), ciprofloxacin (57.8), and amikacin (48.6%). On other hand, chloramphenicol (19.7%), doxycycline (10.8%), ceftazidine (10.8%), erythromycin (6.5%), gentamicin (3.9%), cefotaxime (3.9%), amoxiclav(3.9%), tetracycline (3.9%), vancomycin (3.9%) and cefixime (2.6%) showing the lowest percentages sensitivity. Resistant profiles were determined. A total of 12 different resistotype patterns were obtained; common resistotype were 5 and 11.Conclusions This study shows that there is an increased rate of incidence of Pseudomonas aeruginosa in burns infections and most of these isolates were multi-drug resistant and showed different resistotyping patterns.