BIOFILM FORMATION BY METHICILIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) WITHIN HOSPITAL AND COMMUNITY ACQUIRED URINARY TRACT INFECTIONS

Abstract

Background and objectives Methicillin resistant Staphylococcus aureus is a significant cause of life-threatening human infections, which can switch from planktonic forms (i.e. single cells) to biofilms. Biofilm formation was often-lower susceptibility to antibiotic treatments and development of chronic infections. The study was investigated biofilm formation by methicillin resistant Staphylococcus aureus isolated from patients with urinarytract infection. Meanwhile assess the relationship between biofilm formation and antibiotic resistance.Methods Staphylococcus aureus were isolated and identification by standard methods from urinary tract infections at three teaching hospitals in Erbil city. Methicillin resistant Staphylococcus aureus were detected by PBP2a. Heterogeneity of of methicillin resistantStaphylococcus aureus was determined by efficiency of plating method. Minimum inhibitory concentration of antibiotics was determined by agar dilution method. Biofilm forming abilityof methicillin resistant Staphylococcus aureus was investigated.Results Methicillin resistant Staphylococcus aureus were resistances to 10.92±3.17antibiotics. The percentage of biofilm formation by methicillin resistant Staphylococcus aureus was 82%. Strong biofilm formations were resistance to13.40±2.51 antibiotics, which is statistically higher than biofilm negative (Mean±SD = 6.56±1.51).Conclusions Most methicillin resistant Staphylococcus aureus were biofilm forming. Biofilm formation was correlated with multiple antibiotics resistance and to heterogeneous of methicillin resistant Staphylococcus aureus.