Study of HLA-class II Serotyping and cellular immunity CD4+T, CD8+T cells in Iraqi patients with Rheumatoid arthritis


Rheumatoid arthritis is a complex polygenic disease whose environmental and genetic factors contribute to both of the predisposition and developing of disease. The current study was conducted to determine the frequency of human leukocyte antigen (HLA-DR, -DQ) and correlate to this variation with disease severity and detected on the role of cellular immune response in pathogenesis of rheumatoid arthritis. Fifty blood samples were collected from RA patients and 50 healthy control group with no history of inflammatory arthritis. The HLA class II (DR, DQ) estimated serologically by using micro lymphocytotoxicity test. The result indicated that a highly significant frequencies of HLA-DR4 and DR53 antigens were observed in RA as compared to healthy group (P<0.001). Our results revealed that there are significant higher frequencies of HLA-DQ3 antigen (P<0.001) in RA group as compared to control groups. Additionally, the percentage of CD8+ has been decreased in patients in comparison with healthy group, whereas the percentage of CD4+ T-cell has been slightly increased in patients in comparison with healthy control group with nonsignificant differences. The results of this study proved that nonsignificant correlation which was observed between cellular immune response (CD4 and CD8) among patients.