Immunohistochemical Expression of Ki 67 &CD31 in Iraqi Patients with Astrocytomas, Clinicopathological Studies

Abstract

background: Astrocytomas are the most common primary tumor of the central nervous system, the diagnosis absolutely based on histology has a high interobserver variations and remains problematic even for an experienced neurological pathologist.The objective of this study: To assess the immunohistochemical expression of Ki67 as proliferative markers and CD31 as an endothelial cell marker in astrocytomas interrelated with some clinicopathological parameters (age, gender, site of the tumor, and tumor grade) in Iraqi patients.Materials and methods: In this retrospective study, 41 formaline–fixed paraffin –embedded tissue blocks represent cases of Astrocytomas, The histopathologic diagnosis had been revised and all cases were stained by immunohistochemical technique with Ki 67 & CD 31 antibodies and assessed independently by three pathologists. Values were considered statistically significant when p<0.05.Results: Fibrillary astrocytoma (WHO grade II) was found to be the most common type among astrocytic tumors with the peak age incidence in the second and sixth decades of life, and a slight male predominance had been identified. Parietal location of tumor consider most common site ,There was a significant correlation between the age of the patients and the grade of the tumor on one side & Ki-67 labeling indix, and microvessel density (MVD) detected by CD31 on other side (p<0.05). While no significant correlation with the site and gender. Conclusion: A significant correlation was found between Ki67 labeling indices, and MVD (microvessel density) in one side, and the clinicopathological variables of astrocytomas in other side, Could be used as ancillary methods in the differentiation of borderline grades of astrocytomas.