Evaluation of Soluble L-Selectin (CD 62L)Level in Embryo Culture Media to Embryo Quality and Implantation Rate


AbstractBackground:Although progress was made in an in vitro fertilization (IVF) techniques,the majority of transferred embryos fail to implant. Morphology embryoscoring is the standard procedure for most of IVF centers for choosing the bestembryo, but remains limited since even the embryos classified as (top quality)may not implant. Determining molecular mechanisms of human embryo implantationis an extremely challenging task due to the limitation of materials andsignificant differences underlying this process among mammalian species. L-Selectinand its ligand carbohydrate have been proposed as a system that mediatesinitial adhesion of human blastocysts to the uterine epithelia.Objectives:Quantitative evaluation of soluble L-Selectin molecule in an in-vitroculture media used for in vitro fertilization. Determine cut-off value of solubleL-Selectin quantity to be as qualitative predictor of successful implantationPatients and Methods: This prospective study was undertaken in the High Instituteof Infertility diagnosis and assisted Reproductive Technologies/ Al-NahrainUniversity/ Baghdad/ Iraq, during the period from September2017 to April2018.A total of 74 infertile women were underwent controlled ovarian hyperstimulation for intracytoplasmic sperm injection cycle. Flexible antagonist protocolwas used as ovulation induction protocol in all the cases .culture mediaL-Selectin level were measured on the day of embryo transfer by using Enzymelinked immune sorbent assay (ELISA) for all cases . Comparison in culture mediaL-Selectin was done to all cases.Results: There was no significant difference between pregnant and non-pregnantgroups in age , BMI, and level of basal hormone FSH, LH , AMH and E2 in theday of hCG injection, and significant difference in the level of L-Selectin inpregnant compared to non-pregnant groups.Conclusion: Level of L-Selectin concentration in embryo culture media can beused as predictor of ICSI success