Single‑Nucleotide Polymorphism of Interleukin‑27 Gene: A Risk Factor of Recurrent Pregnancy Loss in Iraqi Women


Background: Studies have been done to investigate the association between a single‑nucleotide polymorphism (SNP) of interleukin‑27 (IL‑27)gene and the recurrent pregnancy loss (RPL). However, different results have been found in different spots of the world. Therefore, more studies are needed to understand the variation in these results. This is the first study that shows the implication of the SNP of IL‑27 gene in RPL. Objective: This study aims to investigate the association between RPL and SNP of gene (−964 A > G) in Iraqi women.Materials and Methods: From September 2013 to September 2014; 100 women, as a control group, and 100 women (with three or moreconsecutive pregnancy loss), as a study group, were recruited to investigate the association between the IL‑27(−964 A > G) SNP and the PRL. The IL‑27(−964 A > G) SNP was determined using polymerase chain reaction‑restriction fragment length polymorphism technique. Genotypeand allele frequencies were compared using Fisher test between the two groups. P < 0.05 is considered to be statistically significant. Results: The age and body mass index were both not significantly different between the two groups. The frequencies of genotypes of this polymorphism in the RPL group were AG (60%), AA (31%), and GG (9%), while these frequencies were AG (21%), AA (68%), and GG (11%) in the control group.The genotype frequencies of the −964 A > G polymorphism was significantly different between the study and the control groups (P = 0.007). The allele frequencies of this polymorphism were A (35%), G (65%) in the RPL group versus A (61%), G (39%) in the control group. The frequencies of A and G alleles in the both groups were not significantly different. Conclusion: IL‑27 (−964 A > G) polymorphism is a risk factor for RPL in a sample of Iraqi women. However, this is different from what has been found in some studies which might implicate other factors in the RPL.