Antimicrobial activity of vaginal Lactobacillus against urogenital pathogens

Abstract

Lactobacillus is one of the most important genera of Lactic Acid Bacteria (LAB). Lactobacilli present in a healthy vagina are part of normal bacterial microbiota and protect the host from urogenital infections by maintaining a low pH. Important features of probiotic lactobacilli are to achieve antagonistic activity against bacterial pathogens because of their capacity to produce lactic acid and other organic acids, H2O2 and bacteriocin. This study aimed to studying the effect of Lactobacillus spp. isolated from healthy vagina as aprobiotic against many clinical bacterial isolates from urogenital tract infection. Susceptibility to Five antibiotics, such as amikacin (AK), Tobramycin (TOB), Pipracillin (PRL), Nalidixic acid (NA) and Gentamycin(GN) was achived on all pathogenic bacteria isolated from urine and vagina. Two fold dilutions of Lactobacillus sp. were prepared (1 × 108, 1 × 104 and 1 × 102) CFU/ml to determine antibacterial activity against urogenital bacteria isolates using agar well diffusion method. N. gonorrhoeae from vagaina was affected by all these dilutions of Lactobacillus sp. with inhibition zones 10, 8 and 8 mm respectively. While inhibition zones of S. aureus and Candida albiccans from vagaina were 12mm in dilution (1 × 104 ) and 6 mm in dilution(108 ) respectively. In addition there was no biological activity of Lactobacillus sp.against bacteria isolated from urine. The effectiveness of natural lactobacilli from vagina consider as a good therapeutics against bacterial illness of humans . We need future study to purification the active component like bacteriocin or other organic acids from this bacteria.