Viability, survivability and tolerability of Rhodococcus pyridinivorans GM3 for phenol concentrations


Microorganisms play a major role in saving our environment by degrading xenobiotic compounds and chemical wastes, which are toxic either in their native or modified form. Bacteria capable of degrading phenol are common and comprise aerobic and anaerobic catabolizing phenol as a sole source of energy and carbon.The bacterial isolate Rhodococcus pyridinivorans GM3 was studied for viability, survivability and tolerability GM3 for different concentrations of phenol in mineral salts medium(MSM). The results showed that R. pyridinivorans GM3 had powerful adaptability to the some conditions of environment, which exhibited that it has a high survivability and tolerability upto 7.0 g/L of phenol concentration in the medium. The tolerance of microorganism to phenol toxicity reduced with increasing concentrations. There was increase in survivability of R. pyridinivorans GM3 on MSM when inoculum size was increased from 1% to 4%.The results suggest that inoculum size led to increase survivability and tolerability. The results of this study clearly demonstrate that presence of phenol in media upto 2.0 g/L does not adversely affect the viability. However, the inhibitory effect of phenol on viability indicated that GM3 could not degrade 2.5 g/L phenol concentration with 1% inoculum size. R. pyridinivoransGM3 exhibited highest potential forphenol concentrations.