The Molecular and Biological Study of the Isolated Bacteriophages Infecting Pseudomonas aeruginosa From Sewage water


Pseudomonas aeruginosa is one of the Multi-Drug-Resistant organisms mostfrequently isolated worldwide. And because of a shortage of new antibiotics, bacteriophages are considered an alternative for its treatment. Previously, P. aeruginosa phages were isolatedbest candidates were chosen based on their ability to form clear plaques and their host range. This work aimed to characterize one of those phages and evaluate the antibacterial activity of isolated bacteriophages in laboratory animal . This studywas carried out during the period from June 2015 to January/ 2016. It included used of P.aeruginosa phage samples collected from sewage water.Fourtype of P.aeruginosaphage wereidentified according to genetic study, However, analysis of the P. aeruginosa phages genome revealed that it was show closed related to NCBI-Blast Pseudomonas phage (KU297675.1) and others The local Pseudomonas phage isolates (F2 FF, E1 EF, and F1 FF) were show as unique isolates and different than NCBI-Blast Pseudomonas phage. The results showed the vast diversity of P. aeruginosaphages may be useful in treatment of infection with antibiotic resistant bacteria.We studied the biological activity of P.aeruginosa phage isolate in the burned Rabbits model.The result showedresponsible for a significant reduction in the bacterial population. The burned mouse model showed an animal survival between 80% and 100%, significantly different from the control animals (0%).All the rabbits completely recovered after 8 days of treatment and showed the absence of signs after 18 days after phage application in treatment.So, considered as a good candidate for phage-therapy.