Biofilm Inhibitory Potential of Westiellopsis prolifica Extract Against Some Pathogenic Microorganisms

Abstract

Abstract- Resistance of microorganisms to many kinds of antibiotics pushtowards using natural products to eliminate the biofilm. In this study, 7different species of algae were identified, 3 species of Chlorophyta and 3 ofCyanophyta. Westiellopsis prolifica is considered a potent organism. Fouriertransform infrared spectroscopy (FTIR) was determined 13 clear bands, andthe main bands were carbohydrate (1200- 900 cm−1), protein (1660 andaround 1540 cm−1) and lipid bands (1740 cm−1). Extracellular crudeacetone extract from W. prolifica better than hexane extract and more efficienton negative gram bacteria than positive gram bacteria. Antibiofilm wasconducted by Congo red Agar and Microtiter plate against bacterial isolates(Staphylococcus aureus, Bacillus subtilis, Streptococcus sp., Shigella sp.,Proteus sp. and Pseudomonas aeruginosa) and two fungi (Aspergillus nigerand Candida albicans). The highest inhibition was against Streptococcus sp.and the remaining ratio of biofilm was 0.7 %, while lower inhibition 33 %against C.albicans. The GC-MS analysis of the purified extract has identifiedmany active compounds, mainly were 4-Trimethyl-1-hexene, Octadecane,Ethylene-14-Pentadecane, n-Hexadecanoic acid, Octadecadienoic acid,Octadecadienoyl chloride, and Phytol