Effect of arginine and selenium with vitamin E in some biochemical properties of Inseminated ewes

Abstract

The study was conducted in one of the fields of the private sector within Basaaer the village of thedistrict of Hit / Anbar at 180 km west of Baghdad, during the period from 17/4/2018 to 30/5/2018.28 ewes with an age of 2-4 years with a weight of 40-50 kg with at least one birth. All ewes weretested using ultrasonography to ensure that they were free of pregnancy before the start of theexperiment. Synchronization of estrus with intravaginal sponges was achieved in ewes, andinseminated with rams at the end of the hormonal program. The rams remained with the femalesfor five days. The ewes were randomly divided into four equal groups (7 ewes in each group). Thefirst group were injected with 160 micro mol / kg body weight and from the fifth day of theinseminated until day 20 of pregnancy The second group was treated by injecting selenium plusvitamin E with (2 ml/head α-Tocopheryl Acetate 68mg/ml Selenium 1.5mg/ml) intramuscular. Thefirst dose was given on day 5 and the second one on day 19 of insemination the early atpregnancy. The third group were injected the arginine with the selenium plus vitamin E emulsion,The fourth group was injected with a physiological saline solution and considered as control group.Blood samples were collected from jugular vein prior to treatment on day 4 of the insemination aswell as on days 10, 15, and 18, to measure the changes in the chemical properties at the early ofpregnancy. The results of the study showed a significant difference (P≤0.05) between thetreatments and control groups. The results showed a decrease in the concentration of urea andALT enzyme as compared with the first group and control. The present study did not show asignificant differences at early of pregnancy in the concentration of cholesterol, total protein,albumin, globulin, glucose, and AST enzyme at the early of pregnancy for ewes. The results alsoshowed a significant difference (P≤0.05) between the different withdrawal periods within the sametreatment in the control and other groups the concentration of ALT enzyme, and total protein.It can be concluded from this study that the use of arginine and selenium with vitamin E improvesmaternal health by reducing the level of urea and ALT enzyme and maintaining pregnancy afterinsemination.E-mail- ibtihajal