PCR technique for detection of Giardia lamblia in microscopically positive stool samples of the patients in Al-Diwaniyah hospital /Iraq.

Abstract

Giardia lamblia is one of the most common human parasites and causes a lengthily course ofnonbacterial diarrhea. Disease outbreaks due to Giardia infection are often attributed to contaminated foodand water supplies. A major problem associated with detection for this organism is the lack of sensitive andreliable methods. PCR has the potential to address many of the limitations. We have performed a PCR-basedmethod for sensitive detection of Giardia. Because the sensitivity of PCR is a function of the efficiency ofDNA extraction from parasite. Giardia samples were collected from stool of infected persons and firstdetected microscopically and the second step used of the PCR technique to ensure the infection,DNA wasextracted and used of a 619 bp conserved fragment related to the DNA was extracted ,and theGL50803_17190 Eukaryotic peptide chain release factor subunit 1[Giardia lamblia ATCC 50803 ]gene wasused as the target for PCR amplification which rarely used. The results suggest the potential utilities of PCRfor sensitive detection of G.Lamblia which isolated from stool of infected persons