Isolation and diagnosis of some skin fungi and identification of their ability to produce enzymes Hemolysins and Protease
Abstract
The present study examined the isolation and diagnosis of dermatophyte fungi from patients attending dermatology consultant of Salahuddin General Hospital and diagnosed by specialized physicians during the period from October 2018 to May 2019 and detection of some virulence factors. Trichophyton and Microsporum were isolated and the most common was Trichophyton simii and Microsporum fulvum, with isolates of 6 and 16.67%, while Trichophyton tonsurans had the lowest infection with 4 isolates, 11.1%. By clinically diagnosing the injuries, there were six clinical forms: Tinea corporis, Tinea manum, Tinea uagium, Tinea pedis , Tinea inguinalis and Tinea capitis. The highest rate was Tinea corporis 25% and the lowest rate was Tinea inguinalis 8.3 %. In this study, the susceptibility of isolated fungi to the production of protease and hemolysins enzymes was identified , Trichophyton mentagrophytes and Trichophyton rubrum showed the highest percentage of hemolysins with 3 isolates at 8.33% each , While Trichophyton simii had the lowest single isolation and 2.70%, Microsporum fulvum and Trichophyton tonsurans were not producing hemolysin. The highest protein decomposition rate for Microsporum fulvum was 6 isolates and 16.67% , The lowest were Trichophyton simii and Trichophyton tonsuran with one isolation and a percentage of 2.7% .
Keywords
Dermatophytes, Hemolysin, Protease, Tinea uagium, Tinea capitis, Tinea pedis, Tinea manum, Tinea corporis, Tinea cruris.Metrics