Effect of Moringa oleifera Extract on Serum Glucose and Interleukin-1, Interleukin-2 and Tumor Necrosis Factor α in Streptozotocin-Induced Diabetic Rats

Abstract

ABSTRACT: Background: Diabetes Mellitus presents a major challenge to healthcare systems around the world. One of the outcomes of diabetes is oxidative stress that is caused by the effect of hyperglycemia. Recent studies indicate that, oxidative agents in diabetes result in many complications such as cardiovascular disease, nephropathy, retinopathy and neuropathy. Aim of the study: This study was designed to evaluate the effect of Moringa oleifera (MOE), in Streptozotocin (STZ)-induced diabetes in wistar rats. Methods: The rats were randomly divided into three groups of 10 animals each: Group I, healthy control; Group II, diabetics; Group III, diabetics treated with MOE (100 mg/ kg) for12 weeks . Diabetes was induced by a single intra-peritoneal injection of Streptozotocin (STZ, 65 mg/kg).Finally, blood samples were collected and analyzed for glucose and interleukin-1(IL-1), interleukin-2(IL-2), and tumor necrosis factor α (TNF-α). Results; result revealed that, oral administration of MO extract caused a significant decrease in serum glucose IL-1, and TNF-α levels(183± 41.10mg/dl), (25.7 ± 1.5 pg/ml), (10.7 ± 2.0pg/mL)respectively , whereas increased in IL-2(5.0 ± 1.92 U/mL) in diabetic rats treated with MO extract when compared to levels of diabetic and control groups (298.2 ± 8.09mg/dl), (97.24 ± 3.21pg/ml), (13.4 ± 2.21pg/mL),and (4.08± 1.17U/mL) respectively. Conclusions: It appears that, Moringa olifera extract had both antihyperglycemic and decreased the activity of IL-1, and TNF-α , and increased IL-2 on STZ-induced diabetic rats. Further studies are needed to determine its protective effects on the other diabetes complications.