Background: The clinical significance of leptin hormone in predicting pregnancy after controlled ovarian hyperstimulation (COH) protocols for intracytoplasmic sperm injection (ICSI) is still poorly understood. Several reports exist on the prospects of hormonal profile in the luteal phase for the diagnosis of early pregnancy.Objectives: This study was designed to investigate the role of follicular fluid leptin in predicting pregnancy in women underwent ICSI cycle.Materials and Methods: This study included 54 women aged 16–44 years who underwent their first ICSI cycles in Baghdad IVF infertility centre in the period between December 2014 and May 2015, they have normal ovulatory cycles, and without any evident endometrial pathology. All patients received mid-luteal long-protocol down-regulation with GnRH- agonist. Each patient was monitored for ovarian follicular development by transvaginal sonography and series of serum E2 level. Women were given human chorionic gonadotropin (hCG), Serum E2 level was estimated on the day of hCG administratin. Intracytoplasmic sperm injection procedure was done by the embryologist. Chemical pregnancy will be defined at the 14th day after embryo transfer by elevated serum β-hCG level, then, progesterone supplementation was continued up to 12 weeks’ gestation.Evaluated Follicular fluid leptin at day of oocyte retrieval.Results: The results of this study showed that 11 women achieved pregnancy(20.37%) and was defined as pregnant group, while 43 women failed in achieving pregnancy(79.63%) who defined as non pregnant group after they underwent their first ICSI –ET treatment cycles. There was no significant difference in follicular fluid Leptin at day of Oocyte retrieval between the pregnant and non-pregnant group. A significan positive correlation between follicular fluid leptin with BMI in pregnant pateints. A significant positive correlation between follicular fluid leptin with BMI in non-pregnant women.Conclusion: Evaluation of follicular fluid leptin level was not a promising diagnostic and prognostic parameter for the prediction of pregnancy in ICSI-ET treatment cycles. Follicular fluid leptin vary between the pregnant patients based on maternal BMI.