Analysis of genetic diversity of clinical bacterial isolates Proteus vulgaris Using RAPD-PCR technique

Abstract

RAPD-Polymerase chain reaction technique was using to analyses genetic variety for fivebacterial isolates Proteus vulgaris which isolated from different sources (urine, stool, otitismedia, wound and burn). Sixty samples were collected from patients and identified five isolatesof P. vulgaris , then the result of the reactions of RAPD technique of these five isolatesdone by using eighteen primers appeared ten primers differences of the number amplifiedbands and different in molecular size, the total number of bands was (131), the number ofmonomorphic bands was (5), while the number of polymorphic bands (126), The highestnumber of polymorphic bands (21 bands) produced by primer OPA-06, while the primer(OPI-16) appeared lowest number of polymorphic bands (2). The primer efficiency rangedfrom 1.52 (primer OPI-16) to 16.03 (primer OPA-06). On the other hand, the discriminatorypower ranged from 1.58 % (primer OPI-16) to 16.66 % (primer OPA-06), Then determinedin this study maximum genetic distance (0.79067) which found between isolates collectedfrom stool and urine, while the minimum genetic distance determined about (0.41505) wasfound between isolates of burns and wounds, The results appeared that the ability of RAPDtechnique to find genetic differences between the five bacterial isolates P. vulgaris and createa unique DNA distinctive bands that can differentiate between five isolates P. vulgari s andthat can be used as a distinctive genetic finger printing for bacteria in epidemiological studies