Association of Catechol‑O‑Methyltransferase Gene Polymorphism with Benign Prostatic Hyperplasia in Babylon Province

Abstract

Background: Benign prostatic hyperplasia (BPH) is a common nonmalignant disorder in elderly men. Objectives: The objective of thepresent study was planned to evaluate the frequency and association of catechol‑O‑methyltransferase (COMT) gene G↔A (Val 158 Met)single‑nucleotide polymorphism (SNP) with BPH in Babylon Province. Materials and Methods: To accomplish this purpose, 146 patientswith BPH and 102 apparently healthy controls were subjected to the study. DNA was extracted from whole blood for all samples. Genotyping ofCOMT gene G↔A (Val 158 Met) SNP was carried out by allele‑specific oligonucleotides‑polymerase chain reaction. Results: Results indicatedthat the homozygous genotype (Met158Met) (AA) of COMT gene G↔A (Val 158 Met) SNP was found to be significantly increase the risk ofBPH by three folds with respect to those of the wild genotype (Val158Val) (GG) of COMT gene G↔A (Val 158 Met) SNP. The heterozygousgenotype (Val158Met) (GA) of COMT gene G↔A (Val 158 Met) SNP was found to be none significantly increase the risk of BPH with respectto those of the wild genotype (Val158Val) (GG) of COMT gene G↔A (Val 158 Met) SNP. The minor allele frequencies (A) of COMT geneG↔A (Val 158 Met) SNP were significantly higher in BPH patients when compared with that of the control group. Conclusions: The COMTgene G↔A (Val 158 Met) SNP is involved in the pathogenesis of BPH.