Study of Biochemistry and Analytical of Metformin as a Suggested Pro-drug for Phosphoamide

Abstract

The aim of this study is to prepare a new phosphoamide pro-drug derived from metformin to minimize the side effect and study the effect of this derivative In vivo. The prepared compound was diagnosed by (Fourier Transform Infrared Radiology FTIR, Nuclear magnetic resonance of the proton1H-NMR, Mass spectroscopy). The effect of the prepared derivative was studied on rabbits, Forty Local rabbits By weights (1000-1800) g and ages (6-8) months were divided randomly into four groups. The first group )G1( consisting of 10 rabbit is a healthy control group that has not been given any substance. The second group (G2) consisted of 10 rabbits which were injected with alloxane150mg / kg B.W intraperitoneally (positive control). The third group (G3) consists of 10 rabbits given 2ml of metformin at a concentration 0.0485M orally by agavage tube. The fourth group (G4) consists of 10 rabbits given 2ml of derivative at a concentration 0.0623 M orally by agavage tube. Blood samples were withdrawn Method stab the24 hours heart after giving the last dose, which lasted for four days, which is the withdrawal on the fifth day and the serum was separated. The results of the statistical analysis of the effect of the compound showed a significant decrease in the activity of aspartate aminotransferase compared with the positive control, and a significant decrease in the concentration of each of glucose, insulin as a result of the compound studied compared with the positive control, and no significant differences in the concentration of each of hormone C- peptide, urea, creatinine and the activity each of dipeptidyl peptidase-4, basal phosphatase, alanine aminotransferase as a result of the effect compared with positive control. There were no significant differences of the effect of the compound in the concentrations of glucose, insulin, hormone C- peptide, urea and creatinine, and the efficacy of the enzyme transporter aminotransferase and dipeptidyl betetidase- 4 and aminotransferase compared with the control group (negative control) except the basal phosphatase enzyme was observed a significant increase compared to negative control.