STUDY THE EFFECT OF ETHANOLIC EXTRACT OF CERATOINA SILIQUA, GLIMEPHAN AND METFORMIN ON SEMEN FLUID QUALITY IN DIABETIC MALE GUINEA PIG INDUCED BY ALLOXAN

Abstract

This study was carried out in an animal house of the Collage of Veterinary Medicine/ University of Basrah, An attempt has been done to induce diabetic by alloxan in male guinea pigs and investigation of the effects of diabetic on semen fluid quality. Moreover, the present study aimed to evaluate the ameliorating effect of ethanolic extract of Ceratonia siliqua, metformin and Glimphan in experimentally induced in diabetic male guinea pigs. The study was applied on (30) adult male guinea pigs, their weight ranged between (800-1000g) and aged between 6-6.5 months. The male guinea pigs were divided randomly into five groups, each group consist of six guinea pig as the following: Group1:- Male guinea pigs at (Negative controls) administrated normal saline3ml orally for 30 days. Group2:- Male guinea pigs at(Positive control) given alloxan (150mg/kg B.W. I.P) for three days and remain for 30 days. Group3:- Male guinea pigs given alloxan (150mg/kg B.W. I.P.) for three days, then treated with Glimphan drug ( 0.1mg/kg orally administration) for 30 days. Group4:- Male guinea pigs given alloxan (150mg/kg I.P.) for three days, then treated with Metformin drug(15mg/kg orally administration) for 30 days. Group5:- Male guinea pigs given alloxan 150mg/kg I.P. for three days, then treated with ethanolic extract of Ceratonia siliqua fruit (500mg/kg B.W. orally administration) for 30days. At the end of experimental period, the blood samples were collected from heart by cardiac puncture, the serum was isolated to be used for the analysis of biochemical parameters such as glucose concentration, FSH, LH and testosterone concentrations. The results showed a significant (P≤0.05) increase in glucose concentration of the serum diabetic male guinea pig group as compared with (-ve) control group, whereas the results were revealed a significant(P≤0.05)decrease of glucose concentration guinea pigs treated with ethanolic extract of Ceratonia siliqua. The results obtained a significant decrease (P≤0.05) in body weight, FSH, LH and testosterone concentrations in serum and semen, sperm motility, sperm concentration, total sperm cell, live-dead sperm and significant (P≤0.05) increase in sperm abnormalities. While guinea pigs that are treated with ethanolic extract of Ceratonia siliqua the showed a significant (P≤0.05) increase in body weight. In addition, this extract improved the reproductive system by significantly increasing FSH, LH and testosterone concentrations in serum and semen, sperm motility, sperm concentration, total sperm cell, live-dead sperm and significant (P≤0.05) decrease in sperm abnormalities of the diabetic male guinea pigs treated with extract as compared with (-ve) control and another treated groups. Histological examination showed many pathological changes in pancreas and testis in diabetic group but in those that are treated with ethanolic extract of Ceratonia siliqua, the histological changes were near the normal status. It is concluded that good anti-diabetic activity, hypoglycemia effect and spermatogenic activities. Based on these results, we suggested the possible utilization of Ceratonia siliqua as a therapy to prevent diabetic and improved the performance of male reproductive system as compared with other treated such as glimephan and metformin drugs.