Histopathological Study of Using Single-layer Simple Continuous and Continuous Lembert Suture Patterns for Systotomy Closure in Rabbits

Abstract

Two techniques of single-layer closure were studied experimentally by created cystotomy in six male rabbits, they separated randomly into two equal groups (A and B). In animals of either groups, cystotomy was initiated experimentally on the dorsal side of the urinary bladder wall through standard midline ventral laparotomy. In group A, the cystotomy wound was closed by single-layer, full thickness, simple continuous pattern, while in group B, the closure was accomplished by single-layer continuous Lambert pattern, applying polydioxanone PDS (3/0) in the two groups. The effectiveness of cystotomy wound healing and a urine-firm seal of the urinary bladder were examined at days 3, 7 and 14 post-operatively by gross changes. In both groups, the macroscopic observation revealed thickening of the bladder wall with adhesion in both groups, the microscopic evaluation showed optimum healing at the site of cystotomy wound sealing on the 14th day post operation despite a slight to moderate adhesions were noted in both groups. The histological evaluation of the urinary bladder biopsies indicated regular epithelization of the mucosal coat and normal arrangement of the muscularis and serosal layers at the location of cystotomy closure were evident in animals of both groups on the 14th day post operation.