Kinetics of α-amylase enzyme inhuman serum

Abstract

Objective: The present study was conducted to study the general characterization of α-amylase (AMS) enzyme by studing of the effect of various factors which affected on the activity of the enzyme (α-amylase ) by caraway method, in control group and patients group who was studied . Method: Twenty five healthy subject were depended in this study, and indicated the normal level of the enzyme, the factors effect of the activity α-amylase enzyme are: pH, temperature, substrate concentration, incubation duration. The evolution of the factors exhibit significant effect on the activity of the enzyme, and the Vmax and Km were found 100,25 mg/dl respectively, in 20 patients with pancreatitis., the Vmax and Km were found in this group 71.42, 30mg/dl.Results: α-amylase activity was observed to be very stable enzyme and effective at pH (5- 7.8), temperature (37,40), 0.06 mg/dl substrate concentration. Vmax and Km were found 25 mg/dl,100 in healthy group but in patients group were found 30 mg/dl,71.42. AMS activity in serum patients of pancreatitis was very stable in pH 7-7.6, temperature 37C° substrate concentration. The results of serum enzyme activity measurement revealed significant (p<0.005) elevation of AMS activity in patients of pancreatitis when compared with those of the healthy subject.