THE USE OF ELECTRONIC BROWSERS FOR VERTEBRATE GENOME AS A SCIENTIFIC BASIS FOR PREDICTING THE RESULTS OF MOLECULAR ANALYZES IN ADVANCE TO DETERMINE THE GENOTYPES OF THE HOLSTEIN COWS AND COMPARING THEM WITH THE RESULTS OF THE PCR-RFLP AND SEQUENCING TECHNIQUES

Abstract

The aim of the study is to use the electronic browsers of vertebrate genome as a new method and scientific basis for predicting the results of molecular analyzes in advance represented by determination of genotypes of the studied genes sites (Leptin and Leptin-R and FABB3) for the Holstein cows on DNA copy or cDNA version and comparing them with the results of the PCR-RFLP and sequencing techniques and previous studies to reach the best and most accurate way to selection animals with distinctive productive traits, in this study 50 Holstein cows were used, in order to separate the DNA and determine the genotypes of the studied gene sites, two genotypes of SNP (rs29004501) studied for leptin gene were found in the studied cow sample (AA and AB), as well as two genotypes of SNP (rs133672995) studied of leptin receptor gene (CC and CT), the FABP3 gene showed three genotypes of SNP (GG, GA and AA), and two SNP (rs383276104) genotypes (GG and GT) with the absence of SNPs (rs136297772 and rs132781540) in the studied cows sample. It is possible to obtain all the information and details of the studied genes fragmentto predict the results before starting laboratory work and compare them with the results of the PCR-RFLP and sequencing techniques and previous studies, as well as the possibility of selection and nomination of genes proposed study by relying on the available data in the gene bank through the use of electronic browsers for genome Vertebrates (National Center for Biotechnology Information (NCBI), the University of California Santa Cruz (UCSC) and the Ensembl Genome Browser), it is the best research method to support research and studies of animal production and future strategies in the field of molecular genetics, and also supports the selective breeding method used by breeders to improve the productive traits of livestock, it enables us to know the number and percentage of genotypes and and allele frequency for the studied genes sites correctly and accurately.