EFFECT OF DIFFERENT COMBINATION OF GROWTH REGULATORS ON THREE POTATO (SOLANUM TUBEROSUM L. ) CULTIVARS FOR GROWING IN VITRO

Abstract

The study was carried out at the laboratory of plant tissue culture - college of Agriculture - University of Basra during 15 /3/2013 to 20 l2l2015 to study the effect of salinity stress on some growth indicators contain in callus produced from three potato varieties (Lizeta, Arnova and Safari) in Vitro. The sprout of the three potato varieties (Lizeta, Arnova and Safari) were cultured in aseptic condition on Murashige and Skoog (MS) full strength media supplemented with IAA and BA at concentration of 1.0 mg / L-1 in addition to GA3 at concentration of 0.1 mg / L-1. The results sammurized at follow: The effect of cultivars and growth regulators results sammaurized: No significant effect on regeneration , while Arnova cultivar was superior in number of shoots reached 2.48 shoot .plant-1. .Lizeta was significantly superior in number of leaves (1.59 leaves.plant-1 ), the combination of hormons (1.0 mg.L-1 of each IAA & BA) was superior in height plant and number of leaves reached 1.6 leaves..plant-1.The combination of Lizeta cultivar with (1.0 mg.l-1 each of BA and IAA affected significantly on number of leaves (2.1 leaves .plant-1.)