Study of Interleukins 13, 18, 27, 32 and 38 Levels in Iraqi Type 2 diabetes with Insulin Resistance.

Abstract

The present study was planned to determine the serum levels of interleukins (IL)- 13, 18, 27, 32 and 38 in type 2 diabetes mellitus (T2DM), as well as, insulin were quantitatively determined in patients and control subjects by means of sandwich ELISA test using commercially available kits. Also body mass index (BMI) and homeostatic model assessment (HOMA-IR) were calculated.Twenty one Iraqi T2DM patients and 21 control subjects matched for age and ethnic background were also included.The median serum level of IL-13, 18, 27, 32 and 38 in patients compared to healthy control (5.12 vs. 4.575, 237.5 vs. 145.9, 278 vs. 368, 205 vs. 143 and 144 vs. 166 pg/ml) respectively, while the median serum level of insulin (11.58 vs. 4.59 μIU/ml) and HOMA-IR (1.8vs. 0.6) was significantly higher in T2DM patients as compared to controls. BMI showed a significant increased in T2DM patients as compared to controls (32.1 vs. 25.6)The receiver operator curve (ROC) analysis of the forthcoming variations revealed the descending order of FSG (0.998), HOMA-IR (0.983), BMI (0.970), FSI (0.972), serum IL-32, 27, 18, 38, and 13 (0.946, 0.810, 0.712, 0.691 and 644) respectively, that showed significant variation. The Spearman’s rank showed a strong correlation to the variables S.Insulin (r=0.986), S.IL-18 (r=0.708), S.IL-38 (r=0.669), S.IL-13 (r=0.624), S.IL-27 (r=0.574),with insulin resistance In conclusion, high level of IL-13, IL18, IL32 and low levels of IL-27, IL-38 were associated with an increased risk of T2DM especially in obese Iraqi diabetics due to Insulin resistance.