Correlation Between Tumor Suppressor Gene P53 With Squamous Cell Carcinoma of Oral Tongue Patients By Using Immunohistochemical assay and InSitu Hybridization Method

Abstract

The incidence and mortality from squemous cell carcinoma of the tongue have increased during the recent years. So, the current study was desinged to investigate the comparative between two methods Immunohistochemistry (IHC) and In Situ Hybridization (ISH), to detect the correlation between tumor suppressor gene (p53) in fourty patients with tongue Squemous cell carcinoma (SCC).The total of 40 specimens were collected during the period between (2010-2012).The histological types involved 22 well differentiated,5 moderately differentiated and 13 poorly differentiated carcinomas.Tumor suppressor gene p53 was detected immunohistochemicaly in nineteen out of fourty (47.5%), whereas, by using insitu hybidization, p53 was detected in 21 out of 40 (52.5%), the positive results for p53 was related in highly significant to each of age, gender, histological stage and grade of the tumor.Its conclude from these results that (IHC) and (ISH) for detection of tumor suppressor gene (p53) was useful in the clinical evaluation of patients with tongue cancer , also the mutations of p53 seemed to be an additional prognostic marker that was associated with well, moderate and poor differentiated of the tumor.