Effect of some strains of lactic acid bacteria and their mixture on the biochemical traits of liver and thyroid gland in male rats with hypothyroidism induced using carbimazole


The current study aims to use different strains of lactic acid bacteria in the treatment of thyroid diseases, Fortified milk containing the selected bacterial isolates L. Plantarum, L. caseiandL. acidophilus and their mixture were used in a dose of 104 experimental animals of 117 male albino rats aged 9-12 weeks at an average weight of 23 g with induced hypothyroidism at a concentration of 0.6 g of carbimazole.1000g-1 rat weight , while control treatment (13 mice) was drank liquid milk only. After the experiment was completed, blood samples were drawn to estimate the efficacy of liver enzymes Glutamate Oxaloacetate Transfer ease (GOT) and Glutamine Pyruvate Transferees (GPT), in addition to estimating thyroid hormones that included (Tryiodotheronin and Tetraiodothyronin) and Thyroid Stimulating Hormone (TSH). In addition, microscopic examination of tissue sections was token from the thyroid gland. The results of thestudy showed a significant increase in the level of liver enzymes in the group of mice affectedwith hypothyroidism induced by carbimazole compared to the control sample, and its return tosignificant increase when feeding with liquid milk containing bacteria under study and itsmixture with a significant increase in the level of thyroid hormones triiodothyronine (T3) andThyroxine (T4) and a significant decrease in the level of (TSH) when feeding with milk fortifiedwith lactic acid bacteria. The results of the histological study of thyroid sections showed thattreatment with carbimazole and hypothyroidism led to degenerative changes in thyroid folliclecells and loss of cellular coordination with infiltration of inflammatory cells, bleaching ofcolloid, and hyperplasia in thyroid follicles.