Biosynthesis and Characterization of Silver Nanoparticles from Pantoea agglomerans and Some of Their Antibacterial Activities Layla Abdulhamid Said*

Abstract

Recently, the biosynthesis of nanoparticles from bacteria have attracted attention, this study hasbeen made for biosynthesize and characterizes silver nanoparticles (AgNPs) from local clinicalisolate Pantoea agglomerans. The ability of those particles to inhibit the virulence factorsbiofilm and hemolysin produced by some local clinical multidrug-resistant human pathogensincluding Acinetobactor haemolyticus, Escherichia coli, Serratiamarcescens and Staphylococcus aureus were investigated by treating all of the test isolates withsub-MIC(16 mg/ml) AgNPs. The AgNPs produced were characterized using Atomic ForceMicroscopy (AFM). Pantoea agglomerans were found to have the ability to synthesize AgNPsat room temperature within 24hrs and were spherical in shape as depicted by AFM. The AgNPsproduced exhibited a potential antibiofilm and hemolysin inhibition activities against testedpathogens