Histological Assessment for Healing of Intraoral Surgical Wounds Produced by Diode Laser 940 nm Versus Surgical Scalpel Blade (An in Vivo study)


The objectives of this study were to compare the wounds healing between two different surgical procedures and to evaluate the efficiency of the diode laser on wound healing after intra-oral incision. Materials and Methods: Experimental study were done on 16 New Zealand rabbits. Two types of wound incision were made in buccal mucosa of each group, each incision was made by using stainless steel scalpel blade NO.15 and other was made by Epic X diode laser. Two rabbits sacrificed of each group at 1st ,3rd ,7th and 14th days after procedures. Results: The histological findings of this study revealed that there was a significant difference for inflammatory cell infiltration in 1st ,3rd , 7th days in two groups, while there was no significant difference at 14th days period. Granulation tissue formation appeared higher in diode group , while scalpel showed less amount of granulation tissue formation . In re-epithelialization of present study at 1st day all section showed nil scores, re-epithelization of oral mucosa appeared significant difference after three days ,and significant difference also at 7th day between diode and scalpel , no significance differences at 14 days in two groups. Conclusions: Diode laser has many advantages for use particularly in oral surgery , due to a better hemostatic ability, but more tissue damage occurs and more inflammatory cell infiltration in site of incision if compared with group that used of surgical scalpel blade