In vivoandin vitrocharacterization of probiotic organisms for theirmicrobial adhesion property isolated from Coconut toddy

Abstract

In current scenario it is a big challenge for keeping good health. The adulterate food we get in the market makes the researcher tothink for safeguarding their digestive system. To solve the problem probiotics which are the live bacteria and yeasts helps in ourgood health, especially our digestive system. To keep our gut healthy the present study has focused on two probiotic organismsnamelyLactobacillus fermentum(KT183369) andBacillus subtilissub sp.Inaquasporium(KR816099) were isolated from co-conut toddy and they were optimized for their probiotic nature. Further, the probiotic bacteria were analysed and have been found toshow good adhesive property towards the host cell. During this study, the adhesive properties of probiotic bacteria could beidentified by using ornamental fish black moley (Poeciliasphenops) and also by Microbial Adhesion To Hydrocarbon assay (MATHassay) using toluene as solvent. The probiotic bacterium showed good adhesive properties in bothin vivoandin vitrostudies usingaquatic fish and its pathogen,Vibrio paraheamolyticusrespectively. Additionally, during this study, the fishes were infected alongwith the probiotic feed to check their mortality rate and the hydrophobicity percentage of the probiotic organisms was alsocalculated using the assay method during.