Influence of aquatic environment on microbiota ofLiopropoma santifish in a local river in Iraq

Abstract

The overall objective of the present study was to evaluate the autochthonous microbiota present in the anterior mucosa (AM),posterior mucosa (PM) of the gut and gill (G) of Santi fish. Total count and total coliform bacteria in different sites in the river (Al-Kamalia) were also assessed. Aerobic colony count (ACC) and total coliform bacteria (TCB) were enumerated using tryptone soyagar and MacConeky agar, respectively. Most probable number (MPN) was employed to assess the coliform bacteria numbers in thewater.Results showed that the mean log ACC and TCB in the AM were 5.97 CFU g1and 5.08 CFU g1, in the PM were 5.77 CFU g1and 4.66 CFU g1and in the G were 5.96 CFU g1and 4.89 CFU g1, respectively. In addition, the mean log ACC in water was6.76 CFU mL1, while the mean log TCB assessed by MPN was 1.5 cell ml1.Staphylococcusspp.,Aeromonas sobria, Aeromonas hydrophilaandEscherichia coliwere isolated from both fish and the watersamples,Citrobacter freundiiwas identified in the anterior mucosa and gill.E. coli, Klebsiella pneumonia, Enterobacterspp.andCitrobacterspp. were the common coliform bacteria isolated from the river.