Response of some pathogenic microorganisms to crude alkaloid of endophytic fungi isolated from Catharanthus roseus

Abstract

Thirteen isolates of endophytic fungi were isolated from leaves and roots of Catharanthus roseus, these fungal isolates had been collected and identified by scientific laboratories of College of Science / Al-Mustansiriyah University in the period from 3rd to 7th of November, 2019. These fungal isolates were Aspergillus niger, Fusarium solani, Cladosporium cladosporioides, Fusarium oxysporum, Mycelia sterilia, Curvularia sp., Alternaria alternate, Aspergillus parasiticus, Aspergillus flavus, Penicillium sp., Aspergillus ochraceus, and two isolates of Aspergillus terreus, the first one was isolated from leaves and the second isolate from stems. In addition to these isolates, eight isolates of common bacterial isolates (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Pseudomonas aeruginosa, E. coli, Salmonella typhi, Shigella sp. Klepsiella Sp.) as well as one yeast isolate (Candida albicans) were obtained from the same laboratories. The antimicrobial ability of the whole fungal extracts were tested separately against the nine selected pathogens by using well diffusion method. The results showed variable response and antimicrobial actions of endophytic fungal extracts against the selected bacteria and yeast. The ranges of actions were ranging between high ability of inhibition to no action against some isolates, which may be due to the difference between the cell wall of gram negative and positive.