Extraction and Assessment of Antioxidative Activity from Wheat Germ oil

Abstract

Iraqi wheat germ oil was extracted by Soxhlet extractor. The percentage of moisture, protein, fat, ash and carbohydrates was estimated in the Iraqi wheat germ. Extract the oil from the germ using a soxhlet extractor. The phenolic content in it was estimated. The antioxidant efficacy of the oil was assessed by estimation of its DPPH (1,1-diphenyl-2-picrylhydrazyl) radical reabsorption, and an estimate of its reduction capacity and its ability to bind to the ferrous ion. Estimate the oil content of vitamin E with High Performance Liquid Chromatography (HPLC) technology. The percentage of moisture, protein, fat, ash and carbohydrates in the wheat germ was 10.13, 26.82, 9.98, 5.12 and 47.95%, respectively. The total content of phenolic compounds and vitamin E in wheat germ oil was 50 g. ml and 310 mg. 100g, respectively, increases the ability of the oil to scavenging of radical DPPH, its reductive capacity and its ability to bind ferrous ion to increase the concentrations used.