The current study was carried out to investigate some immunohistopathological effects of bovine bile salts against Salmonella typhimurium. In laboratory animal thirty albino rabbits of both sex that ages ranged between (10-12) months were divided randomly into three groups ,the first group immunized orally by 0.5ml (5mg/ml) and the second group immunized orally with 0.3ml (3mg/ml) of bile salts in zero time after two weeks the same doses repeated against Salmonella typhimurium, then delayed type hypersensitivity test was made on these groups and compared with the third group "that had been inoculated with 0.5 ml of sterile PBS as a control group at day 27 post immunization using soluble bile salts. At day 30 half number of animals from each group were scarified. Then challenge dose by 1ml of salmonella typhi were injected intraperitoneally (I/P) for the remaining half of all study groups ,then after three days they scarified. Samples of blood were collected from each group to ELIZA analysis for determine the level of IgG in serum in order to obtain their tissues for histopathological .The results showed that marked necrotic lesion, together with blood vessel congestion accompanied by hyperplasia with hypertrophy goblet cell of intestine and thickness of splenic capsule due to mononuclear cells (MNCs) infiltration. While the immunized groups with bile salts revealed presence of granulomatous lesion in liver with lymphoid hyperplasia and slight megakaryocyte proliferation in spleen. Finally, the results showed that the bile salts was highly immunogenic against Salmonella typhimurium and its toxins and this may be related to the fact that the one of component of bile salts deoxy cholic acid in their structure which has the ability of destroyed effect on salmonella and their toxins in the rabbits tissues.