Vestige radiosources radiation on Leishmania donovanii

Abstract

Abstract The objective of this treatise in order to determine insinuation of radiosources rays on Leishmania donovanii. In this descriptive study design of 10 Leishmania donovanii cross-sectional study design, collected from Al-Karamah hospitals in Baghdad from different patients.The parasite was diagnosed based on biochemical tests and depending on its cultural characteristics by utilizing a special medium N199 semi soild medium for growth leishmania. Study populations of (10) Leishmania donovanii in order to treatise vestige radiation by cultivated on M199 media at 25̊ C for five days to reach the stationary-phase culture, then culture was centrifuged (5000 rpm for 10 min). The pellet was suspended in 150 ml of sterile normal saline ,then 1 ml of this solution was exposed to different radioactive radiations, comparison to control group (without exposure),rediluted and inoculated in M199 media, then cultivated within test tube containing distilled water 5ml, then exposing to an different radiosources including CS137, Sr90 and CO60, with activity 1-10 µci with different radiation doses for different peroids 1 hrs.