Histological study to determination of carbon nanotube particles in testis of ducks and it affection on apoptosis during spermatogenesis

Abstract

This study designed to identification carbon nanotube particles (CNTs) and their cytological effects on the testicular tissue of birds breading in areas with carbon pollution. Twelve of mal domestic ducks were collected from pure farms and zones exposed to air pollution by carbon remnants of factories (six birds for each group). Histological stains were used to distinguish general structure of testes samples, in addition dual staining (acridine orange stain/ ethidium bromide stain) were used to show apoptosis event. The morphological design of carbon nanoparticles was determined by scanning electron microscope (SEM) procedure. The SEM results revealed to two morphological styles of CNTs: The ultrafine carbon black (UFCB) as ball-shaped and multiwalled carbon nanotubes (MWCNTs) as fiber shape were scattered through testicular tissue. Moreover, Fluorescence microscopy results evidenced the early apoptotic stage (as yellow color) and advanced of apoptotic stage (as orange color) in the seminiferous tubule epithelium and in interstitial tissue of testes birds that living in polluted zones. This study concluded that testis morbidity of birds living in the polluted farms related to the rout of administration and the accumulative dose of carbon pollutants from near brick factories.