Genetic detection of some virulence factors for urinary tract infections isolated from Diuresis for people with thalassemia

Abstract

This study was conducted for the purpose of detecting some virulence factorgenes of the bacterial species isolated from the urine of children with Thalassemia,160 urine samples were collected from healthy children and 46 urine samples werecollected from children with Thalassemia , Results showed that 84 sample (52.5%) ofthe total samples studied gave positive bacterial growth, while 76 (47.5%) gives negativesgrowth on media cultivation in healthy children samples.In children with Thalassemiahave shown positive growth of 36 samples (78.3%) and 10 samples (21.7%) werenot give any growth .the purpose of detecting some virulence factors for some studiedisolates, DNA was isolated for all isolates. Four isolates were taken, represented bytwo isolates of E. coli and two isolates of Entero.faecalis., one of them isolated fromhealthy children and the other one from Thalassemia infected children with use 3specialized primers for each bacteria.