Investigate Pseudomonas aeruginosa resistant to antibiotics and studying the antibacterial effect of Rosemary oil at different concentrations

Abstract

In this work, 110 clinical samples were collected, including samples of wounds, burns,otitis and urinary tract infection, from some hospitals in Baghdad Governorate, fromNovember 2019 to January 2020. All samples were cultured on differential culture media,as 53 P. aeruginosa bacterial isolates were identified using microscopic examination andbiochemical test. (33) Isolates belonging to Pseudomonas aeruginosa were confirmed byVITEK_2 system. The polymerase chain reaction technique was used to detect the (16srRNA) and (toxin A) genes. The results of the electrophoresis of the results of the polymerasechain reaction to diagnose the specific gene (16S rRNA) showed the expectedgene bundles with the size of (956) base pair for 33 isolates. Addition, The electrophoresisresults for the gene detection (toxin A) showed bundles with the size of (352) basepair of 81% of these isolates. The sensitivity of these bacterial isolates to some antibioticswas studied. Most of them were resistant to Piperacillin, Amikacin, Gentamicin and Meropenemby 71%, 63% and 60%, respectively. Whereas were resistant to Imipenem by 27%and Ceftazidime by 33%.The chemical analysis of the oily extract for the rosemary plant(Rosmarinus officinalis) was carried out using gas chromatography-mass spectrometry(GC-MS), and the essential compounds were β_ trance ocimene 33.27%, Camphor 12.77%,Camphene 12.37%, Benzene methanol 7.22% and linalool ester 2.46%. The antibacterialactivity of volatile oil extract for Rosemarinus Officinalis plant was evaluated and reachedto (6.25 mg.ml-1), therefore, we concluded from this study that rosemary plant oils haveanti-bacterial activity against P.aerugginosa.