Development of high-performance liquid chromatography using simplex method for the simultaneous determination of some decongestant drugs


Using high-performance liquid chromatography method, three decongestant drugs, chlorpheniramine maleate (CPM), paracetamol (Para) and phenylephrine hydrochloride (PPH) were quantitatively determined in pharmaceutical preparations. Using two methods. In both methods, isocratic displacement and a mobile phase consisting of (acetonitrile: a buffer solution consisting of 0.01 M dipotassium phosphate and monopotassium phosphate), a temperature of 38 °C, a wavelength of 228 nm, a volume of injected substance 20 μl, and a separation column of NUCLEODUR®. C18 (250 x 4.6 mm id) ec were used, In the first method (univariable method), it was found that the pH function was 7.8, the flow rate was 1.0 ml/min and the mobile phase ratio was 50:50 acetonitrile: Buffer solution), In the second method; the multivariate method (simplex method), it was found that the best pH function is 7.7 and Flow rate 1.0 ml/min and the mobile phase ratio 48:52 (acetonitrile: Buffer solution), and the results were close, as the retrieval was (95.18 -105.00) with a detection limit and a quantitative limit (0.092 -0.301 mcg/ml) and (6.440 -2.107 mcg/ml) respectively.