Effect of Steroids and Black Seed Oil on Hydrogen PeroxideConcentration and pH of Expired Breath Condensate inAsthmatic Patients

Abstract

Asthma is a chronic inflammatory disease of airway, with increased worldwide prevalence. This study
was conducted the for evaluation the effect of different treatment regimens on expired breath condensate
hydrogen peroxide concentration and pH. Patients and methods: Thirty two patients (17 male, 15 fernale),
with asthma were examined during exacerbation. Asthma was diagnosed according to American Thoracic
Society Criteria. The pH level was increased significantly following ffeatment with prednisolone. In
addition, expired breath condensate hydrogen peroxide in patients with acute attack reduced significantly
(p<0.0001) following treatment with prednisolone. Black seed oil treatment reduced expired breath
condensate HzOz conientration from 1.29 pM (95% Cl, 1.08-1.50) as baseline values to 0.56 pM (95%
CI,0.45-0.67) following treatment. Furthermore, pH of expired breath condensate increased from 5'54
(g5o' Cl, 4.g7-6.11) at 6aseline to 6.8 (95% CI, 6.28-7.32) following treatment course of back seed oil'
Thus the treatment course of black seed oil significantly reduced expired breath condensate HuOz
concentration (p< 0.0001) and increased exhaled pH (P< 0.01). For the groups that were treated with oral
prednisolone or black seed oil, there were a highiy significant inverse correlation among expired breath
condensate Hzoz with pH, Exhaled Hzoz wiitr pEvt predicted percent and positive correlation for
exhaled pH with FEVI predicted percent. Steroids and back seed are with activity to reduce expir^ed
breath condensate hydrogen peroxide concentration and pH. This may indicate that they may be used for
asthma treatment.