Separation and determination of some water-solublevitamins in pharmaceutical preparation by IP RP-HPLC

Abstract

A reversed phase(RP) HPLC with ion-pair reagent is used for simultaneous
determinations of water-soluble vitarnins (nicotineamide, nicotinic acid, lolic acid,
riboflavin (B2) and thiamin (B1)) in pharmaceutical preparations. The vitamins were
analyzed using ZORBAX Cs (25cm x 4.6mm i.d) analytical column, 6-8pm particle
size, by isocratic elution with a mobile phase consisted of 20%methanol, buffered
with 0.05M KH2PO4 (pH 3.5) , and 3x10-rM l-octanesulfonic acid as an ion-pair
reagent at a flow rate of (1.Oml/min.). The UV-Visible detection of the separated
vitamins was made at 254nm for all water-soluble vitamins. The precision and
accuracy of method were checked by calculating RSD% and relative error E%, which
where found to be reasonable A RSD% between (0.5-4.76) 0% and relative error
lhetween (0.05-4.93)%.The limit of detection range was lound to be (0.0244,
d'.0+:opglmt;.