Engineering of a Multi-Epitope Subunit Vaccine Against SASRS-CoV-2 Through the Viroinformatic Approach

Abstract

The COVID-19 outbreak has infected millions of people worldwide, but no vaccine has been discovered to combat itefficiently. This research aims to design a multi-epitope vaccine using highly efficient B- and T-cell epitopes from theSARS-CoV-2 Surabaya isolate through a viroinformatic approach. First, the putative epitopes were linked together todevelop tertiary structures and then docked with toll-like receptor 4 (TLR-4) that demonstrated a robust interaction witha low eigenvalue of 4.816138 e¡06. Furthermore, the structure's high immunogenic response was observed and successfully cloned into the expression vector pET28a (þ). This implies that the designed vaccine can prove effective incombating SARS-CoV-2.