PRUIFICATION OF DNASE PRODUCED BY LOCAL STREPTOMYCES ISOLATES

Abstract

ABSTRACTThe DNase enzyme which produced from local (Streptomyces sp.SH-10) waspurified by fractionation by aqueous two phase system followed gel filtrationthrough Sephacryl S-200 by two steps. The final purification folds was 4.33andthe yield of the enzyme was 28.8% . The molecular weight of the purified enzymedetermined by gel filtration was 19950, The optimum pH for enzyme activity andstability were 7.0 and 6.5-7.5 respectively, and the optimum temperature forenzyme activity was 35oC .___________________________________________________