Effect of Sulfacetamide Compound in Growth and Initiation of Nigella sativa L. Stem's Callus

Abstract

The study included the effect of sulfacetamide on the growth of seedlings and callus derived from Nigella sativa L. stems. This compound was added to the Murashige and Skoog ( MS )medium at concentrations of (10-2,10-3,10-4,10-5, 10-6) Molar. The average of roots length and the rate of germination in seeds, treated with sulfacetamide before planting, differed with the concentration of sulfacetamide used. The high concentration (10-2 M) led to death of the seeds. The results revealed decrease in the fresh weight of callus with decrease in the cellular contents of proteins, nucleic acids(DNA,RNA)and folate extracted from callus which was grown on MS medium containing 10-6 M of 2,4-D and various concentrations of sulfacetamide (10-3,10-4, 10-5,10-6M). On the other hand, the high concentration of sulfacetaminde (10-2 M) led to death of the initiated cells of callus.