Effect of methanolic extract for (leaves and roots) of Bacopa monniera L. aerial plant parts on the growth of some bacteria and fungi

Abstract

he project was conducted to study the inhibitory activity of methanolic extract for aerial parts (leaves and roots) of Bacopa monniera L. on some bacterial species represented by: Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Escherchie coli, Klebsellia sp. Shigella sp., by using the Agar-well diffusion method for estimating the Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC) as well as two type of fungi, Aspergillus niger and Aspergillus flavus, the fungi used to study the antifungal activity of plant extract. The results showed a difference in the effect for the types of bacteria as the value of (MIC) for the types Bacillus cereus, Shigella sp. and Klebsellia sp. Is a (100, 200, 200) while there was no effect of the extract against other bacterial species under study.As for the fungi testing, the extract has a clear effect by measuring the mean of growth diameter for fungi, where the growth diameter of the fungus A. niger 1.03 cm at the concentration 50mg/ml while the diameter became 0.5 cm at the 300 mg/ml concentrate in the first day, and in the seventh day of the experiment, the growth diameter reached 7.03 cm at the 50 mg/ml concentrate but the concentration 300mg/ml were 6.4 cm compared with control, which amounted to 7.6 cm. As for the fungi A. flavus growth diameter (1.1 , 0.46) cm at concentrations (50, 300) mg/ml in the first day of the experiment, in the seventh day the diameter was (4.73,3.9) cm at concentrations (50, 300) mg/ml respectively, while the diameter of growth has been different at the other concentrations and within test days.