Study of antimicrobial activity of some active secondary compounds for Viola odorata on growth for some types of bacteria


The study was conducted to track the inhibitory activity of methanolic, flavinoid and alkaloid extracts of Viola odorata with concentrations (1, 1.5, 2, 2.5, 3) mg/ml on some bacterial species represented by S. aureus, Bacillus subtilis, Pseudomonas aeruginosa, E.coli and Klesiella sp, by using disk-diffusion method and estimated inhibitory activity of extracts on bacteria by measuring the Diametric of zone inhibition. The results showed a difference in the effect of each extract for the types of bacteria, the alkaloid gave a strong inhibitory ability against all types of bacteria compared with the methanolic extract and flavinoid respectively; this activity was enhanced with the increasing of extracts concentrations and the (3) mg/ml concentration gave highest activity, Pseudomonas aeruginosa was the most effective one, where the diameter of zone inhibition (25) mm at this concentration while E.coli was the least testing bacteria effective by the concentration and the diameter of zone inhibition was (10) mm. Methanolic extract showed antimicrobial highest activity on S. aureus, the diameter of zone inhibition was (21) mm with no antimicrobial activity on Pseudomonas aeruginosa and Klesiella sp, while the flavinoid showed highest activity on Pseudomonas aeruginosa, the diameter of zone inhibition was (13) mm with no antimicrobial activity against Klesiella sp. and E.coli.